Microassay for the detection of elastase activity in the gingival crevice.
Identifieur interne : 000508 ( Main/Exploration ); précédent : 000507; suivant : 000509Microassay for the detection of elastase activity in the gingival crevice.
Auteurs : J M Herrmann [Allemagne] ; J R Gonzáles ; R H Boedeker ; J. Vonholdt ; J. MeyleSource :
- Journal of clinical periodontology [ 0303-6979 ] ; 2001.
Descripteurs français
- KwdFr :
- Adulte (MeSH), Analyse de régression (MeSH), Coumarines (MeSH), Exsudat gingival (enzymologie), Fluorimétrie (méthodes), Gingivite (diagnostic), Gingivite (enzymologie), Humains (MeSH), Indicateurs et réactifs (MeSH), Indice parodontal (MeSH), Leukocyte elastase (analyse), Leukocyte elastase (métabolisme), Mâle (MeSH), Méthode en double aveugle (MeSH), Spécificité du substrat (MeSH), Statistique non paramétrique (MeSH), Tests enzymatiques en clinique (MeSH), Études prospectives (MeSH).
- MESH :
- analyse : Leukocyte elastase.
- diagnostic : Gingivite.
- enzymologie : Exsudat gingival, Gingivite.
- métabolisme : Leukocyte elastase.
- méthodes : Fluorimétrie.
- Adulte, Analyse de régression, Coumarines, Humains, Indicateurs et réactifs, Indice parodontal, Mâle, Méthode en double aveugle, Spécificité du substrat, Statistique non paramétrique, Tests enzymatiques en clinique, Études prospectives.
English descriptors
- KwdEn :
- Adult (MeSH), Clinical Enzyme Tests (MeSH), Coumarins (MeSH), Double-Blind Method (MeSH), Fluorometry (methods), Gingival Crevicular Fluid (enzymology), Gingivitis (diagnosis), Gingivitis (enzymology), Humans (MeSH), Indicators and Reagents (MeSH), Leukocyte Elastase (analysis), Leukocyte Elastase (metabolism), Male (MeSH), Periodontal Index (MeSH), Prospective Studies (MeSH), Regression Analysis (MeSH), Statistics, Nonparametric (MeSH), Substrate Specificity (MeSH).
- MESH :
- chemical , analysis : Leukocyte Elastase.
- chemical , metabolism : Leukocyte Elastase.
- chemical : Coumarins, Indicators and Reagents.
- diagnosis : Gingivitis.
- enzymology : Gingival Crevicular Fluid, Gingivitis.
- methods : Fluorometry.
- Adult, Clinical Enzyme Tests, Double-Blind Method, Humans, Male, Periodontal Index, Prospective Studies, Regression Analysis, Statistics, Nonparametric, Substrate Specificity.
Abstract
BACKGROUND, AIMS
A new microassay for the detection of elastase activity (EA) in gingival crevicular fluid (GCF) has been established. GCF was collected with Periopaper strips and quantified in a Periotron.
METHODS
Enzyme activity was measured in a microtiter plate reader, using a fluorometric assay. To ensure quality and precision of the assay, recovery rates were determined at different activities with a recovery of >90%. In a 2nd step, stability of the enzyme was investigated during storage at room temperature, +4 degrees C, -22 degrees C, -88 degrees C. GCF samples retained elastase activity of almost 100% after a storage of 3 days at -22 degrees C. In a group of 12 healthy volunteers, elastase activity was assayed throughout an 18 day experimental gingivitis protocol.
RESULTS
Median activity increased from 481 microU/microl at baseline to 1444 microU/microl at day 18, which was accompanied by the development of the signs of gingivitis. The increase of EA during the experimental phase of the study was highly significant (p<0.001) and correlated well with the increasing severity of gingivitis.
CONCLUSION
The data suggest that elastase activity in GCF is an excellent quantitative measure of gingival inflammation.
DOI: 10.1034/j.1600-051x.2001.280105.x
PubMed: 11142664
Affiliations:
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Vonholdt</name></author><author><name sortKey="Meyle, J" sort="Meyle, J" uniqKey="Meyle J" first="J" last="Meyle">J. Meyle</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:11142664</idno><idno type="pmid">11142664</idno><idno type="doi">10.1034/j.1600-051x.2001.280105.x</idno><idno type="wicri:Area/Main/Corpus">000526</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000526</idno><idno type="wicri:Area/Main/Curation">000526</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000526</idno><idno type="wicri:Area/Main/Exploration">000526</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Microassay for the detection of elastase activity in the gingival crevice.</title><author><name sortKey="Herrmann, J M" sort="Herrmann, J M" uniqKey="Herrmann J" first="J M" last="Herrmann">J M Herrmann</name><affiliation wicri:level="1"><nlm:affiliation>Department of Periodontology, Dental School, University of Giessen, Germany. jens.m.herrmann@dentist.med.uni-giessen.de</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Periodontology, Dental School, University of Giessen</wicri:regionArea><wicri:noRegion>University of Giessen</wicri:noRegion><wicri:noRegion>University of Giessen</wicri:noRegion><wicri:noRegion>University of Giessen</wicri:noRegion></affiliation></author><author><name sortKey="Gonzales, J R" sort="Gonzales, J R" uniqKey="Gonzales J" first="J R" last="Gonzáles">J R Gonzáles</name></author><author><name sortKey="Boedeker, R H" sort="Boedeker, R H" uniqKey="Boedeker R" first="R H" last="Boedeker">R H Boedeker</name></author><author><name sortKey="Vonholdt, J" sort="Vonholdt, J" uniqKey="Vonholdt J" first="J" last="Vonholdt">J. Vonholdt</name></author><author><name sortKey="Meyle, J" sort="Meyle, J" uniqKey="Meyle J" first="J" last="Meyle">J. Meyle</name></author></analytic><series><title level="j">Journal of clinical periodontology</title><idno type="ISSN">0303-6979</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult (MeSH)</term><term>Clinical Enzyme Tests (MeSH)</term><term>Coumarins (MeSH)</term><term>Double-Blind Method (MeSH)</term><term>Fluorometry (methods)</term><term>Gingival Crevicular Fluid (enzymology)</term><term>Gingivitis (diagnosis)</term><term>Gingivitis (enzymology)</term><term>Humans (MeSH)</term><term>Indicators and Reagents (MeSH)</term><term>Leukocyte Elastase (analysis)</term><term>Leukocyte Elastase (metabolism)</term><term>Male (MeSH)</term><term>Periodontal Index (MeSH)</term><term>Prospective Studies (MeSH)</term><term>Regression Analysis (MeSH)</term><term>Statistics, Nonparametric (MeSH)</term><term>Substrate Specificity (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte (MeSH)</term><term>Analyse de régression (MeSH)</term><term>Coumarines (MeSH)</term><term>Exsudat gingival (enzymologie)</term><term>Fluorimétrie (méthodes)</term><term>Gingivite (diagnostic)</term><term>Gingivite (enzymologie)</term><term>Humains (MeSH)</term><term>Indicateurs et réactifs (MeSH)</term><term>Indice parodontal (MeSH)</term><term>Leukocyte elastase (analyse)</term><term>Leukocyte elastase (métabolisme)</term><term>Mâle (MeSH)</term><term>Méthode en double aveugle (MeSH)</term><term>Spécificité du substrat (MeSH)</term><term>Statistique non paramétrique (MeSH)</term><term>Tests enzymatiques en clinique (MeSH)</term><term>Études prospectives (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Leukocyte Elastase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Leukocyte Elastase</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Coumarins</term><term>Indicators and Reagents</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Leukocyte elastase</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Gingivitis</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Gingivite</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Exsudat gingival</term><term>Gingivite</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Gingival Crevicular Fluid</term><term>Gingivitis</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Fluorometry</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Leukocyte elastase</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Fluorimétrie</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Clinical Enzyme Tests</term><term>Double-Blind Method</term><term>Humans</term><term>Male</term><term>Periodontal Index</term><term>Prospective Studies</term><term>Regression Analysis</term><term>Statistics, Nonparametric</term><term>Substrate Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Analyse de régression</term><term>Coumarines</term><term>Humains</term><term>Indicateurs et réactifs</term><term>Indice parodontal</term><term>Mâle</term><term>Méthode en double aveugle</term><term>Spécificité du substrat</term><term>Statistique non paramétrique</term><term>Tests enzymatiques en clinique</term><term>Études prospectives</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND, AIMS</b></p><p>A new microassay for the detection of elastase activity (EA) in gingival crevicular fluid (GCF) has been established. GCF was collected with Periopaper strips and quantified in a Periotron.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>Enzyme activity was measured in a microtiter plate reader, using a fluorometric assay. To ensure quality and precision of the assay, recovery rates were determined at different activities with a recovery of >90%. In a 2nd step, stability of the enzyme was investigated during storage at room temperature, +4 degrees C, -22 degrees C, -88 degrees C. GCF samples retained elastase activity of almost 100% after a storage of 3 days at -22 degrees C. In a group of 12 healthy volunteers, elastase activity was assayed throughout an 18 day experimental gingivitis protocol.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Median activity increased from 481 microU/microl at baseline to 1444 microU/microl at day 18, which was accompanied by the development of the signs of gingivitis. The increase of EA during the experimental phase of the study was highly significant (p<0.001) and correlated well with the increasing severity of gingivitis.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>The data suggest that elastase activity in GCF is an excellent quantitative measure of gingival inflammation.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11142664</PMID><DateCompleted><Year>2001</Year><Month>03</Month><Day>01</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>31</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0303-6979</ISSN><JournalIssue CitedMedium="Print"><Volume>28</Volume><Issue>1</Issue><PubDate><Year>2001</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Journal of clinical periodontology</Title><ISOAbbreviation>J Clin Periodontol</ISOAbbreviation></Journal><ArticleTitle>Microassay for the detection of elastase activity in the gingival crevice.</ArticleTitle><Pagination><MedlinePgn>31-7</MedlinePgn></Pagination><Abstract><AbstractText Label="BACKGROUND, AIMS" NlmCategory="OBJECTIVE">A new microassay for the detection of elastase activity (EA) in gingival crevicular fluid (GCF) has been established. GCF was collected with Periopaper strips and quantified in a Periotron.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Enzyme activity was measured in a microtiter plate reader, using a fluorometric assay. To ensure quality and precision of the assay, recovery rates were determined at different activities with a recovery of >90%. In a 2nd step, stability of the enzyme was investigated during storage at room temperature, +4 degrees C, -22 degrees C, -88 degrees C. GCF samples retained elastase activity of almost 100% after a storage of 3 days at -22 degrees C. In a group of 12 healthy volunteers, elastase activity was assayed throughout an 18 day experimental gingivitis protocol.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Median activity increased from 481 microU/microl at baseline to 1444 microU/microl at day 18, which was accompanied by the development of the signs of gingivitis. The increase of EA during the experimental phase of the study was highly significant (p<0.001) and correlated well with the increasing severity of gingivitis.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The data suggest that elastase activity in GCF is an excellent quantitative measure of gingival inflammation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Herrmann</LastName><ForeName>J M</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Department of Periodontology, Dental School, University of Giessen, Germany. jens.m.herrmann@dentist.med.uni-giessen.de</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gonzáles</LastName><ForeName>J R</ForeName><Initials>JR</Initials></Author><Author ValidYN="Y"><LastName>Boedeker</LastName><ForeName>R H</ForeName><Initials>RH</Initials></Author><Author ValidYN="Y"><LastName>Vonholdt</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author 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Elastase</NameOfSubstance></Chemical><Chemical><RegistryNumber>OCY3JCT44X</RegistryNumber><NameOfSubstance UI="C028743">7-amino-4-methylcoumarin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>D</CitationSubset><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004796" MajorTopicYN="N">Clinical Enzyme Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003374" MajorTopicYN="N">Coumarins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004311" MajorTopicYN="N">Double-Blind Method</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005470" MajorTopicYN="N">Fluorometry</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005883" MajorTopicYN="N">Gingival Crevicular Fluid</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005891" MajorTopicYN="N">Gingivitis</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007202" MajorTopicYN="N">Indicators and Reagents</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019272" MajorTopicYN="N">Leukocyte Elastase</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010512" MajorTopicYN="N">Periodontal Index</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011446" 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IdType="doi">10.1034/j.1600-051x.2001.280105.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="Boedeker, R H" sort="Boedeker, R H" uniqKey="Boedeker R" first="R H" last="Boedeker">R H Boedeker</name><name sortKey="Gonzales, J R" sort="Gonzales, J R" uniqKey="Gonzales J" first="J R" last="Gonzáles">J R Gonzáles</name><name sortKey="Meyle, J" sort="Meyle, J" uniqKey="Meyle J" first="J" last="Meyle">J. Meyle</name><name sortKey="Vonholdt, J" sort="Vonholdt, J" uniqKey="Vonholdt J" first="J" last="Vonholdt">J. Vonholdt</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Herrmann, J M" sort="Herrmann, J M" uniqKey="Herrmann J" first="J M" last="Herrmann">J M Herrmann</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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